Dissolution of organic matter with two different types of sodium hypochlorite

Abstract

INTRODUCTION: The therapeutic endodontic success depends not only on the instrumentation of the root
canal but also on proper cleaning and disinfecting of the canal system. Disinfection is achieved through the
irrigation of the canal and the most widely used irrigation solution is sodium hypochlorite (NaOCl), since
it has bactericidal and bacteriostatic properties, low surface tension, alkaline pH, but mainly because it is
the only irrigation solution with the ability to dissolve organic tissue. It is presented in different
concentrations from 0.5% to 6%, and different commercial brands for dental and domestic use.
OBJECTIVE: To compare two commercial forms of NaOCl to dissolve organic matter at different times
of exposure.
MATERIALS AND METHODS: we prepared 20 pieces of bovine muscle tissue, which were submerged
for 5, 10, 15, 20 minutes in a NaOCl solution. The sample was divided into 2 groups (n=10) Group 1: dental
5.5% NaOCl and Group 2: domesctic 5.5% NaOCl. Each piece of meat was initially weighed on an
electronic scale (Ohyo MP-300) and recorded in an Excel table. Subsequently, it was submerged in 10ml.
of NaOCl for 5 minutes. It was removed from NaOCl and immersed in physiological solution to eliminate
and inactivate the irrigation solution. It was dried with absorbent paper and weighed on the electronic
balance (Ohyo MP-300). This procedure was repeated at 10, 15 and 20 minutes, renewing the irrigation
solution to simulate the clinical conditions and avoid saturation. In addition, the total dissolution time of
the piece of meat will be recorded. The data collected was registered and assessed by means of the analysis
test with the variable of a factor (ANOVA).
RESULTS: The percentage of dissolution of organic matter with NaOCl for dental use was similar to that
for domestic use at 5, 10 and 15 minutes. At 20 minutes Tedequim dissolved 63.8% organic matter and
Ayudín 69%. The differences were not statistically significant.
CONCLUSION: Under the conditions of this study, it was concluded that both NaOCl Tedequim and
Ayudín dissolved the organic matter in a similar time, being the domestic use of easy access and lower cost.